$@%"%0%m%P%/%F%j%"7A49K!(J

$@86M}!'%"%0%m%/%F%j%&%`$N(JTi$@%W%i%9%_%I(JVir$@NN0h$K?"J*$N9g@.$9$k%U%'%N!<%k2=9gJ*(J($@%"%;%H%7%j%s%4%s$J$I(J)$@$,:nMQ$9$k$H!"(JTi$@%W%i%9%_%I$+$i(JT-DNA$@NN0h$,@Z$j=P$5$l!"$$$/$D$+$N2aDx$r7P$F?"J*:YK&$N@w?'BN(JDNA$@$KAH$_9~$^$l$k!#%$%M$J$I$NC1;RMU?"J*$O!"$3$N%U%'%N!<%k2=9gJ*$r@8@.$7$J$$$N$G!"%"%0%m%/%F%j%&%`$N$C46@w$N$O%"%;%H%7%j%s%4%s$rE:2C$9$k$3$H$,%]%$%s%H$G$"$k!#(J

$@;nLt!'(J

  1. $@G]CO!'%"%0%m%/%F%j%&%`G]M\G]CO!'(JYEP$@G]CO!"(JAB$@G]CO(J
    $@?"J*G]M\G]CO!'%+%k%9M6F3G]CO(J(N6Cl)$@!"A*H4G]CO!J(JN6SE$@!K(J
    $@%"%0%m%/%F%j%&%`7|ByG]CO(J(AA)$@!":FJ,2=G]CO(J(MSRE)$@!"6&B8G]M\G]CO(J(N6CO)$@!"8!DjG]CO!J(JMSHF$@!K(J
  2. $@$@%+%k%Y%K%7%j%s(J(Carbenicillin,$@%U%!%$%6!<@=LtCm$@%O%$%0%m%^%$%7%s(J(HygromycinB, Boehringer Mannheim, Cat No.843555):$@7A49%+%k%9!"?"J*A*H4MQ!#(J50mg/ml$@$NG;EY$G(JPBS(phosphate bufferd saline)$@$KMO2r$7$?>uBV$G;THN$5$l$F$*$j!"$3$l$r(J1ml$@$:$DJ,Cm$7$F!<(J20$@!n$KJ]B8$9$k!#(J
  3. $@%"%;%H%7%j%s%4%s(J(3,5`-Dimethoxy-4`-hydroxy-acetophenone, Aldrich,Cat No.D13.440-6):10mg/ml$@$NG;EY$G(JDMSO(Dimethyl Sulfoxide)$@$KMO2r$7!"
$@J}K!!'(J
  1. $@$@)!40=O.7?Lb@"$j5!$K$+$1!"Lb$r$@)"(J40ml$@$N$@)#%/%j!<%s%Y%s%AFb$G%S!<%+!<$NMO1U$r$@)$%T%s%;%C%H$G2$7!"%7%c!<%l$N<~$j$r%5!<%8%+%k%F!<%W$G%7!<%k$9$k!#(J
    $@)%(J60$@&L(Jmol/m_s,$@#1#6;~4V8w<~4|(J/8$@;~4V0E4|$N>r7o2<$G!"(J25$@!n!"(J3$@=54VG]M\$9$k!#(J
  2. $@%+%k%9$NA0G]M\$H%"%0%m%P%/%F%j%&%`$NG]M\(J
    $@)!M6F3(J3$@=54V$N$@)"%"%0%m%P%/%F%j%&%`$N%/%j%;%m!<%k%9%H%C%/$+$iLG6]$7$?$D$^ML;^$G6]BN$r$@%"%0%m%P%/%F%j%&%`$N46@w$H6&B8G]M\(J
    $@)!(JAB$@G]CO$GA}?#$7$?%"%0%m%P%/%F%j%&%`$rLG6]$7$?Lt$5$8$G(J2$@!A(J3$@2s=q$-$@)"7|By1U$rLG6]%7%c!<%l$KF~$l$k!#(J
    $@)#(J1$@%7%c!<%lJ,$NA0G]M\$7$?%+%k%9$r%a%C%7%eIU$-E{$KF~$l!"(J1.5$@!A(J2$@J,4V%a%C%7%eIUE{8l$H7|By1U$K?;DR$9$k!#(J
    $@)$?;DR8e!"%a%C%7%eIU$-E{$rLG6]$7$?%Z!<%Q!<%?%*%k$N>e$KCV$$$FM>J,$J?eJ,$r=|5n$9$k!#(J
    $@)%6&B8!J(JN6CO$@!K$K(J16$@%+%k%9(J/$@%7%c!<%l$GCV>2$7!"%5!<%8%+%k%F!<%W$G%7!<%k$7$F(J28$@!n!"0E=j2<$G(J3$@F|4VG]M\$9$k$H!"6]BN$,%+%k%9$r$&$C$9$i$HJ$$&$h$&$KA}?#$9$k!#(J
  3. $@%"%0%m%P%/%F%j%&%`$N=|5n$HA*H4(J
    $@)!(J500mg/l$@$G%+%k%Y%K%7%j%s$rF~$l$?LG6]?e@v>t1U$rLG6]%7%c!<%l(J3$@8D$K(J35ml$@$:$DF~$l$k!#(J
    $@)"%+%k%9$r%a%C%7%eIU$-E{$K(J1$@%7%c!<%lJ,(J(16$@%+%k%9(J)$@F~$l!"@v>t1U$NF~$C$?%7%c!<%l$K?;DR$7!"%a%C%7%eIU$-E{$r:81&$K:Y$+$/?6$C$FCf$N%+%k%9$r@v>t$9$k!#(J3$@8D$N%7%c!<%l$K=g$K?;DR$7$F%+%k%9$+$i%"%0%m%P%/%F%j%&%`$r=|5n$9$k!#(J
    $@)#%a%C%7%eIU$-E{$rLG6]$7$?%Z!<%Q!<%?%*%k$N>e$KCV$-!"M>J,$J?eJ,$r=|5n$9$k!#(J
    $@)$A*H4G]CO!J(JN6SE$@!K$K(J16$@%+%k%9(J/$@%7%c!<%l$GCV>2$7!"%5!<%8%+%k%F!<%W$G%7!<%k$7$F(J25$@!n!"L@=j2<$G(J3$@=54VG]M\$9$k!#(J
  4. $@:FJ,2=(J
    $@)!$9$Y$F$N%+%k%9$r:FJ,2=G]CO!J(JMSRE$@!K$K#9%+%k%9(J/$@%7%c!<%l$G0\?"$9$k!#(J
    $@)"%5!<%8%+%k%F!<%W$G%7!<%k$7$F(J25$@!n!"L@=j2<$G:FJ,2=$7$F$/$k$^$GG]M\$9$k!#(J
  5. $@8!Dj$HBQ@-8GBN$N$=$N8e$N@80i(J
    $@)!:FJ,2=8GBN$r8!DjG]CO!J(JMSHF$@!K$KCV>2$7!"%O%$%0%m%^%$%7%sBQ@-$r8!Dj$9$k!#BQ@-$G$"$l$PH/:,$7!"Ln@87?$HF1MM$N@80i$r<($9!#Hs7A49BN$O?7$7$$:,$,?-D9$;$:(J1$@=54V$[$I$G8O;`$9$k!#(J
    $@)"%7%c!<%l0lGU$K?"J*BN$,@80i$7$?$i!"38$r3+$1$l?e$rF~$l!"(J4$@!A(J5$@F|4VFk2=$5$;$k!#(J
    $@)#JD:?7O29<<$N%]%C%H$K0\?"$9$k!#(J