■最近の論文と過去の代表的な原著論文(英語論文)

Mutations in the SF1-U2AF59-U2AF23 complex cause exon skipping in Schizosaccharomyces pombe
Noriko Haraguchi, Tomoko Andoh, David Frendewey and Tokio Tani
J. Biol. Chem., 282, 2221-2228 (2007)

Participation of XPB/Ptr8p, a component of TFIIH, in nucleocytoplasmic transport of mRNA in fission yeast
Fumitaka Mizuki, Takeshi. Namiki, Hiroshi Sato, Hiromi Furukawa, Tadao Matsusaka, Yasumi Ohshima, Ryoko Ishibashi,
Tomoko Andoh and Tokio Tani
Genes to Cells,12, 35-47 (2007)

Visual screening for localized RNAs in yeast revealed novel RNAs at the bud-tip
Tomoko Andoh, Yukiko Oshiro, Sachiko Hayashi, Hideki Takeo and Tokio Tani
Biochem. Biophys. Res. Commun, 351, 999-1004 (2006)

Imaging of single mRNA molecules movement within a living cell nucleus
Hisashi Tadakuma, Yo Ishihama, Toshiharu Shibuya, Tokio Tani and Takashi Funatsu
Biochem. Biophys. Res. Commun, 344, 772-779 (2006)

Nucleocytoplasmic transport of fluorescent mRNA in living mammalian cells :
Nuclear mRNA export is coupled to ongoing gene transcription
Kazuaki Tokunaga, Toshiharu Shibuya, Yo Ishihama, Hisashi Tadakuma,Miyuki Ide, Minoru Yoshida, Takashi Funatsu, Yasumi Ohshima and Tokio Tani
Genes to Cells, 11, 305-317 (2006)

Single molecule imaging of mRNA splicing
Yo Ishihama, Hisashi Tadakuma, Tokio Tani and Takashi Funatsu
Nucleic Acids Symp. Ser., 49, 209-210 (2005)

Hsp16p is required for thermotolerance in nuclear mRNA export in fission yeast Schizosaccharomyces pombe
Jun-ichi Yoshida and Tokio Tani
Cell Structure and Function, 29, 125-138 (2005)

The fission yeast ptr1+ gene involved in nuclear mRNA export encodes a putative ubiquitin ligase
Tomoko Andoh, Abul Kalam Azad, Asako Shigematsu, Yasumi Ohshima and Tokio Tani
Biochem. Biophys. Res. Commun, 317, 1138-1143 (2004)

The nucleolus is involved in mRNA export from the nucleus in fission yeast
Takashi Ideue, Abul Kalam Azad, Jun-ichi Yoshida, Tadao Matsusaka, Mitsuhiro Yanagida, Yasumi Ohshima and Tokio Tani
J. Cell Sci., 117, 2887-2895 (2004)

A mutation in the gene involved in sister chromatid separation causes a defect in nuclear mRNA export in fission yeast
Abul Kalam Azad, Takashi Ideue, Yasumi Ohshima and Tokio Tani
Biochem. Biophys. Res. Commun., 130, 176-181 (2003)

Mutation in the prp12+ gene encoding a homologue of SAP130/SF3b130 causes differential inhibition of pre-mRNA splicing and arrest of cell-cycle progression in Schizosaccharomyces pombe
Yasuaki Habara, Seiichi Urushiyama, Yasumi Ohshima and Tokio Tani
RNA, 7, 671-681 (2001)

The dhp1+ gene encoding a putative nuclear 5'-3' exoribonuclease is required for proper chromosome segregation in fission yeast
Takeo Shoubuike, Kazuo Tatebayashi, Tokio Tani, Shoji Sugano and Hideo Ikeda
Nucleic Acids Res.,29, 1326-1333 (2001)

Fission yeast Toi1p, which interacts with the small GTPase Ran, is required for mitosis-to-interphase transition and poly (A)+ RNA metabolism
Kazuo Tatebayashi, Tokio Tani and Hideo Ikeda
Genetics, 157, 1513-1522 (2001)

Fission yeast Prp4p kinase regulates pre-mRNA splicing by phosphorylating a non-SR-splicing factor
Wiebke Schmidt, Kathrin Richert, Thomas Groァ, Yasuaki Habara, Tokio Tani and Norbert Kaefer
EMBO Reports, 2, 35-41 (2001)

(過去の代表的論文)

Characterization of the ptr6+ gene in fission yeast: A possible involvement of a transcriptional coactivator TAF in nucleocytoplasmic transport of mRNA
  Toshiharu Shibuya, Satomi Tsuneyoshi, Abul Kalam Azad, Seiichi Urushiyama, Yasumi Ohshima and Tokio Tani Genetics, 152, 869-880 (1999)

Isolation and molecular characterization of mRNA transport mutants in Schizosaccharomyces pombe
  A. K. Azad, T. Tani, N. Shiki, S. Tsuneyoshi and Y. Ohshima  Mol. Biol. Cell, 8, 825-841 (1997)

Nucleolar accumulation of poly (A)+ RNA in heat shocked yeast cells: Implications of nucleolar involvement in mRNA transport
  T. Tani, R.J. Derby, Y. Hiraoka and D.L. Spector  Mol. Biol. Cell, 6, 1515-1534 (1995)

mRNA-type introns in U6 small nuclear RNA genes: implications for the catalysis in pre-mRNA splicing
  T. Tani and Y. Ohshima  Genes & Dev. vol.5, 1022-1031 (1991)

The gene for the U6 small nuclear RNA in fission yeast has an intron
  T. Tani and Y. Ohshima  Nature vol.337, 87-90 (1989)

Identification of a novel class of elastase isozyme, human pancreatic elastase III, by cDNA and genomic gene cloning
  T. Tani, J. Ohsumi, K. Mita and Y. Takiguchi  J. Biol. Chem. , 263, 1231-1239 (1988)

Molecular cloning and characterization of a gene for rat U2 small nuclear RNA
  T. Tani, N. Watanabe-Nagasu, N. Okada and Y. Ohshima J. Mol. Biol., 168, 579-594 (1983)

その他の英語原著論文



■最近の代表的な和文総説等

分子細胞生物学辞典(第2版)
 スプライシングに関する項目を中心に改訂及び執筆
 東京化学同人、印刷中(2007)

スプライシング
 谷時雄
 生物物理学ハンドブック(分担執筆)、印刷中 (2007)

スプライシングとmRNA核外輸送
  水城史貴、谷時雄
  「酵母のすべて」、シュプリンガーフェアラーク東京、印刷中 (2007)

酵母研究におけるアイソトープの活用
  水城史貴、萬年太郎、谷時雄
  「RIを使用した実験法」、細胞工学、vol. 26、No.1、92-97 (2007)

mRNA核外輸送の蛍光イメージングと核内構造
  谷時雄
  蛋白質核酸酵素「 細胞核の世界」、Vol.51、2197-2199(2006)

mRNA核内運動の1分子イメージング
  船津高志、谷時雄
  ファルマシア、vol.42、 803-806(2006)

RNA蛍光イメージングで拓く細胞核研究のフロンティア
  谷時雄、安東知子
  Pharma Vision News No.7, 23-29 (2006)

イーストトラフィック:mRNA細胞内輸送のしくみと制御
  谷時雄、安東知子
  実験医学、vol.22、2447-2453 (2004) 

mRNA核外輸送の視覚化
  多田隈尚史、船津高志、谷時雄 
  蛋白質核酸酵素、vol.48、2133-2141(2003)

mRNA情報発現の制御機構と核の機能構造
   谷 時雄
   細胞工学、vol. 21、1174-1178(2002)

snRNAとスプライシング  
   谷 時雄、坂本 博  
   「RNA研究の最前線」、シュプリンガー・フェアラーク、p.101-117 (2000)

mRNAの核外輸送に関与する遺伝子群
  谷時雄、大島靖美
  蛋白質核酸酵素、増刊号「細胞核研究の最先端」、vol 44、1897-1906 (1999)

mRNA前駆体スプライシングと細胞内制御ネットワーク:酵母変異株の解析から
  谷時雄
  細胞工学 vol. 17, No.10, 1504-1511 (1998)

mRNAの細胞内分布およびタンパク質の核移行
  谷時雄
  酵母ラボマニュアル:酵母分子細胞生物学実験法、p.227-254、 シュプリンガー・フェエラーク社 (1998)

分裂酵母におけるmRNA核外輸送:核小体の関与と熱ショックストレスによる輸送阻害
  谷時雄
  蛋白質核酸酵素、増刊号「見る技術」、共立出版 vol. 42, No.7, p1193-1200 (1997)

mRNAの輸送と局在:細胞内交通の手段と信号
  谷時雄
  細胞工学、vol.15、No.11、p1595-1603 (1996)

mRNA前駆体のスプライシング反応機構
  谷時雄
  生化学、第67巻、第8号、p.1010-1031 (1995)

その他の和文総説等

 



■平成13年度研究費

科学研究費補助金
  特定領域研究(B)(2) (谷/代表)
    「mRNAの核外輸送機構に関する研究」
  基盤研究(B) (谷/分担)
    「細胞内mRNAのプロセシングと輸送の1分子蛍光イメージング」


平成13年度 三菱財団研究助成 (谷/代表)
    「
mRNA核/細胞質間輸送の制御による発生調節機構に関する研究」

 

■平成14年度研究費

科学研究費補助金
  特定領域研究(2) (谷/代表)
    「mRNAの核外輸送機構に関する研究」


  特定領域研究(1)(谷/分担):RNA情報発現系の時空間ネットワーク
    「リン酸化によるスプライシングとmRNA輸送の制御機構」

  若手研究(B) (安東/代表)


■国際学会での発表(過去5年間)

1. Isolation and characterization of novel mRNA export mutants in S. pombe
Takashi Ideue, Mari Kubota, Abul Kalam Azad, Mitsuhiro Yanagida, Yasumi Ohshima, Tokio Tani
The second international fission yeast meeting, March, Kyoto, Japan (2002)

2. NUCLEAR EXPORT OF mRNA IN FISSION YEAST
Tokio Tani, Takashi Ideue, Fumitaka Mizuki, Sin Hwang, Jun-ichi Yoshida and Yasumi Ohshima
The second international fission yeast meeting, March, Kyoto, Japan (2002) (invited talk)

3. A mutation in the gene encoding a homologue of human XPB/ERCC3 causes a defect of mRNA export
from the nucleus
Fumitaka Mizuki, Takashi Namiki, Hiromi Furukawa, Yasumi Ohshima, and Tokio Tani
The second international fission yeast meeting, March, Kyoto, Japan (2002)

4. A PUTATIVE S. POMBE HOMOLOGUE FOR RNPS1 IS INVOLVED IN PRE-mRNA SPLICING
AND mRNA EXPORT FROM THE NUCLEUS
Sin Hwang, Akila Mayeda, Yasuaki Habara, David Frendewey, Yasumi Ohshima and Tokio Tani
The second international fission yeast meeting, March, Kyoto, Japan (2002)

5.Ptr7p, A NOVEL ATP/GTP BINDING PROTEIN, IS REQUIRED FOR BIDIRECTIONAL TRANSPORT
OF MACROMOLECULES BETWEEN THE NUCLEUS AND THE CYTOPLASM
Asako Shigematsu, Jun-ichi Yoshida, Yasumi Ohshima and Tokio Tani
The second international fission yeast meeting, March 25th-30th, Kyoto, Japan (2002)

6.Characterization of the S. pombe homologue for RNPS1: A functional relationship between spRNPS1 and PRP3p in mRNA export
Sin Hwang, Akila Mayeda, Yasuaki Habara, David Frendewey, Yasumi Ohshima and Tokio Tani
Eukaryotic mRNA processing meeting, CSHL, NY, USA, 2001

7. Nucleocytoplasmic transport of fluorescent mRNA in living cells: active gene transcription is
required for efficient export of injected mRNA
T. Shibuya, Y. Ishihama, H.Tadakuma, T.Funatsu, T. Tani
Eukaryotic mRNA processing meeting, CSHL, NY, USA, 2001

8. Molecular mechanisms of mRNA transport from the nucleus to the cytoplasm
Tokio Tani (invited talk)
US-Japan Joint Symposium on Chemistry-Biology Interface, Kumamoto Japan, 2001

9. Participation of a homologue of RAD25/ERCC3, a component of TFIIH, in nucleocytoplasmic transport of mRNA in fission yeast
Takeshi Namiki, Hiromi Furukawa, Yasumi Ohshima and Tokio Tani
Fifth Annual Meeting of the RNA society "RNA2000", Madison, USA, 2000

10. Mutation in the prp12+ gene causes gene-specific inhibition of pre-mRNA splicing and
arrest of cell-cycle progression in Schizosaccharomyces pombe
Yasuaki Habara, Seiichi Urushiyama, Yasumi Ohshima1 and Tokio Tani
Dynamic organization of nuclear function, Cold Spring Harbor, NY, USA, 2000

11. Imaging of Single mRNA Molecules Moving within a Nucleus of Living Cells
H. Tadakuma, T. Shibuya, Y. Ishihama, J. Atoh, Y. Habara, T. Tani, and T.Funatsu
Biophysical Society 45th Annual Meeting, Boston, USA, 2000

12. Nucleocytoplasmic transport of mRNA in fission yeast (invited talk)
Tokio Tani International MBSJ Satellite Symposium/CREST-P&P, Fukuoka, Japan, 1999

13. Nucleocytoplasmic transport of mRNA in fission yeast: characterization of temperature sensitive mutants defective in mRNA export (invited talk)
T. Tani The 1st international fission yeast meeting, Edinburgh, UK, 1999

14. The prp12+ gene required for pre-mRNA splicing encodes a homologue of a human spliceosome-associated factor, SAP130/SF3b
Y. Habara, S. Urushiyama, T. Tani and Y. Ohshima
The 1st international fission yeast meeting, Edinburgh, UK, 1999

15. Characterization of the ptr6+ gene in fission yeast: A possible involvement of a transcriptional coactivator TAF in nucleocytoplasmic transport of mRNA
T. Shibuya, S. Tsuneyoshi, A.K. Azad, S. Urushiyama, Y.Ohshima and T.Tani RNA'99, Edinburgh, UK, 1999

16. Ptr7p, a novel protein with an GTP/ATP binding motif, is required for bidirectional nucleocytoplasmic transport
T.Tani, T. Shibuya, S. Urushiyama, S. Tsuneyoshi and Y.Ohshima RNA'99, Edinburgh, UK, 1999

17. A mutation in the S. pombe ptr7+ gene causes a defect in nucleocytoplasmic transport of mRNA and nucleolar fragmentation: Implication for involvement of the nucleolus in mRNA transport
T. Tani, S. Tsuneyoshi, A.K. Azad and Y. Ohshima
Eukaryotic mRNA Processing Meeting, Cold Spring Harbor, NY, USA, 1997

18. A novel role of the ubiquitin system in mRNA transport
A.K. Azad, T. Tani and Y. Ohshima RNA '97, Banff, Canada, 1997

19. Identification and characterization of the genes involved in nucleocytoplasminc transport of mRNA in Schizosaccharomyces pombe (invited talk)
T. Tani International Oji Seminar, Hakone, Japan, 1996

20. Isolation and characterization of temperature sensitive mutants defective in mRNA transport in Schizosaccharomyces pombe
T. Tani, A.K. Azad, T. Akimatsu, S. Tsuneyoshi and Y. Ohshima
RNA meeting, Madison, Wisconsin, USA, 1996

 

最近の国内・国際学会における発表はこちらへ



■特許関連(三共バイオサイエンス研究所勤務時代)

1)ヒト膵臓エラスターゼIIIA   新規動脈硬化防止剤
  特許番号 2004853、出願人 三共株式会社

2)ヒト膵臓エラスターゼIIIB  新規動脈硬化防止剤
  特許番号 2004854  出願人 三共株式会社

3)新規ヒト膵臓トリプシン 医薬
 特許番号 2501307  出願人 三共株式会社

4)ヒト膵臓エラスターゼI  新規動脈硬化防止剤
  特開昭62-253381  出願人 三共株式会社

5)ブタ膵臓エラスターゼII 新規動脈硬化防止剤 
  特開昭61-249387  出願人 三共株式会社

6)ヒト膵臓エラスターゼIIA 新規動脈硬化防止剤
  特開昭62-130686  出願人 三共株式会社

  その他

 


日本 RNA 学会ホームページ


前に戻る